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Image Search Results
Journal: British Journal of Cancer
Article Title: Progressive resistance of BTK-143 osteosarcoma cells to Apo2L/TRAIL-induced apoptosis is mediated by acquisition of DcR2/TRAIL-R4 expression: resensitisation with chemotherapy
doi: 10.1038/sj.bjc.6601021
Figure Lengend Snippet: Effect of chemotherapeutic agents on the expression of DR4 and DR5. ( A ) Analysis of Apo2L/TRAIL receptor mRNA expression in the BTK-143 osteosarcoma cells, assessed by semiquantitative RT–PCR analysis, as described in the Materials and Methods. Expression of the housekeeping gene, GAPDH, served to normalise for starting mRNA. Right panel shows the pattern of Apo2L/TRAIL receptor expression for late-passage untreated and ETP-treated BTK-143 cells. Left panel: Fluorimager-generated images of PCR products for DR4 and DR5 shown in the upper panel were quantitated using IMAGEQUANT software (Molecular Dynamics, Sunnyvale, CA, USA). ( B ) Flow cytometric data of cell surface expression of Apo2L/TRAIL receptors shows increased expression of DR4 and DR5 following treatment with ETP (heavy line) compared with untreated cells (grey line). The 1B5 isotype-matched (IgG1) murine monoclonal antibody was used as a negative control (light line).
Article Snippet: The relative amounts of the PCR products were determined by quantifying the intensity of bands using a
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Generated, Software, Negative Control